USP9X sirna

USP9X siRNA (Human)

Cat. Num. AAA8227109

• Gene Names: USP9X; FAF; FAM; DFFRX; MRX99
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP9X; USP9X siRNA (Human); DFFRX; FAM; USP9; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Deubiquitinating enzyme FAF-X; Fat facets in mammals; hFAM; Fat facets protein-related. X-linked; Ubiquitin thioesterase FAF-X; Ubiquitin-specific protease 9. X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9X sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: USP9X siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,554

• Applicable Applications for USP9X sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human USP9X gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP9X sirna

siRNA to inhibit USP9X expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 145309309
• NCBI GeneID: 8239
• NCBI Accession #: NP_001034679.2
• NCBI GenBank Nucleotide #: NM_001039590.2
• UniProt Accession #: Q93008; O75550; Q8WWT3; Q8WX12
• NCBI Official Full Name: probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 3
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 9, X-linked
• NCBI Official Symbol: USP9X
• NCBI Official Synonym Symbols: FAF; FAM; DFFRX; MRX99
• NCBI Protein Information: probable ubiquitin carboxyl-terminal hydrolase FAF-X
• UniProt Protein Name: Probable ubiquitin carboxyl-terminal hydrolase FAF-X
• Protein Family: Probable ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP9X
• UniProt Synonym Gene Names: DFFRX; FAM; USP9; hFAM
• UniProt Entry Name: USP9X_HUMAN

NCBI Description

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP9X: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin. Widely expressed in embryonic and adult tissues. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 3.4.19.12; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: growth cone; membrane; apical part of cell; cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: mitosis; transcription initiation from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transcription, DNA-dependent; axon extension; in utero embryonic development; hippocampus development; neuron migration; female gamete generation; negative regulation of transcription from RNA polymerase II promoter; cerebellar cortex structural organization; post-embryonic development; chromosome segregation; BMP signaling pathway; cell division; transforming growth factor beta receptor signaling pathway; gene expression

Disease: Mental Retardation, X-linked 99