UBA5 sirna

UBA5 siRNA (Human)

Cat. Num. AAA8241132

• Gene Names: UBA5; THIFP1; UBE1DC1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBA5; UBA5 siRNA (Human); UBE1DC1; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; UBA5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UBA5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 348

• Applicable Applications for UBA5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UBA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBA5 sirna

siRNA to inhibit UBA5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13376212
• NCBI GeneID: 79876
• NCBI Accession #: NP_079094.1
• NCBI GenBank Nucleotide #: NM_024818.3
• UniProt Accession #: Q9GZZ9; Q96ST1; A6NJL3; D3DNC8
• Molecular Weight: 38,537 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway