UBA2 sirna

UBA2 siRNA (Human)

Cat. Num. AAA8236813

• Gene Names: UBA2; ARX; SAE2; HRIHFB2115
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBA2; UBA2 siRNA (Human); SAE2; UBLE1B; SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UBA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 544

• Applicable Applications for UBA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UBA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBA2 sirna

siRNA to inhibit UBA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4885649
• NCBI GeneID: 10054
• NCBI Accession #: NP_005490.1
• NCBI GenBank Nucleotide #: NM_005499.2
• UniProt Accession #: Q9UBT2; O95605; Q59H87; Q6IBP6; Q9NTJ1; Q9UED2; B3KWB9
• Molecular Weight: 60,839 Da
• NCBI Official Full Name: SUMO-activating enzyme subunit 2
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 2
• NCBI Official Symbol: UBA2
• NCBI Official Synonym Symbols: ARX; SAE2; HRIHFB2115
• NCBI Protein Information: SUMO-activating enzyme subunit 2
• UniProt Protein Name: SUMO-activating enzyme subunit 2
• Protein Family: Ubiquitin-activating enzyme
• UniProt Gene Name: UBA2
• UniProt Synonym Gene Names: SAE2; UBLE1B
• UniProt Entry Name: SAE2_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; protein sumoylation; cellular protein metabolic process; SMT3-dependent protein catabolic process; post-translational protein modification