TTC8 sirna

TTC8 siRNA (Human)

Cat. Num. AAA8226127

• Gene Names: TTC8; BBS8; RP51
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TTC8; TTC8 siRNA (Human); BBS8; Tetratricopeptide repeat protein 8; TPR repeat protein 8; Bardet-Biedl syndrome 8 protein; TTC8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TTC8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 196

• Applicable Applications for TTC8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TTC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TTC8 sirna

siRNA to inhibit TTC8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 571026683
• NCBI GeneID: 123016
• NCBI Accession #: NP_001275710.1
• NCBI GenBank Nucleotide #: NM_001288781.1
• UniProt Accession #: Q8TAM2; Q67B97; Q86SY0; Q86TV9; Q86U26; Q8NDH9; Q96DG8; A6NFG2; B3KWA5
• Molecular Weight: 21,712 Da
• NCBI Official Full Name: tetratricopeptide repeat protein 8 isoform D
• NCBI Official Synonym Full Names: tetratricopeptide repeat domain 8
• NCBI Official Symbol: TTC8
• NCBI Official Synonym Symbols: BBS8; RP51
• NCBI Protein Information: tetratricopeptide repeat protein 8
• UniProt Protein Name: Tetratricopeptide repeat protein 8
• UniProt Gene Name: TTC8
• UniProt Synonym Gene Names: BBS8; TPR repeat protein 8
• UniProt Entry Name: TTC8_HUMAN

NCBI Description

This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

TTC8: The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Defects in TTC8 are the cause of retinitis pigmentosa type 51 (RP51). It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in TTC8 are the cause of Bardet-Biedl syndrome type 8 (BBS8). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 14q31.3

Cellular Component: centrosome; cytosol; photoreceptor connecting cilium; cilium

Molecular Function: protein binding

Biological Process: fat cell differentiation; axon guidance; protein transport; sensory processing; regulation of protein localization; sensory cilium biogenesis; organelle organization and biogenesis; establishment of anatomical structure orientation; multicellular organism growth; olfactory bulb development; sensory perception of smell; cilium biogenesis

Disease: Retinitis Pigmentosa 51; Bardet-biedl Syndrome 8