TRIM25 sirna

TRIM25 siRNA (Human)

Cat. Num. AAA8218223

• Gene Names: TRIM25; EFP; Z147; RNF147; ZNF147
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM25; TRIM25 siRNA (Human); EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; TRIM25 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM25 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 630

• Applicable Applications for TRIM25 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM25 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM25 sirna

siRNA to inhibit TRIM25 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68160937
• NCBI GeneID: 7706
• NCBI Accession #: NP_005073.2
• NCBI GenBank Nucleotide #: NM_005082.4
• UniProt Accession #: Q14258
• Molecular Weight: 70,973 Da
• NCBI Official Full Name: E3 ubiquitin/ISG15 ligase TRIM25
• NCBI Official Synonym Full Names: tripartite motif containing 25
• NCBI Official Symbol: TRIM25
• NCBI Official Synonym Symbols: EFP; Z147; RNF147; ZNF147
• NCBI Protein Information: E3 ubiquitin/ISG15 ligase TRIM25
• UniProt Protein Name: E3 ubiquitin/ISG15 ligase TRIM25
• Protein Family: E3 ubiquitin/ISG15 ligase
• UniProt Gene Name: TRIM25
• UniProt Synonym Gene Names: EFP; RNF147; ZNF147
• UniProt Entry Name: TRI25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.

Protein type: EC 6.3.2.n3; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; defense response to virus; negative regulation of interferon type I production