TRIM13 sirna

TRIM13 siRNA (Human)

Cat. Num. AAA8203344

• Gene Names: TRIM13; CAR; LEU5; RFP2; DLEU5; RNF77
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM13; TRIM13 siRNA (Human); LEU5; RFP2; RNF77; E3 ubiquitin-protein ligase TRIM13; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; Ret finger protein 2; Tripartite motif-containing protein 13; TRIM13 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for TRIM13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM13 sirna

siRNA to inhibit TRIM13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55953112
• NCBI GeneID: 10206
• NCBI Accession #: NP_001007279.1
• NCBI GenBank Nucleotide #: NM_001007278.2
• UniProt Accession #: O60858; Q5UBW0; Q5W0U8; Q5W0U9; Q9BQ47; Q9C021; B2RB49
• Molecular Weight: 47,333 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM13 isoform 2
• NCBI Official Synonym Full Names: tripartite motif containing 13
• NCBI Official Symbol: TRIM13
• NCBI Official Synonym Symbols: CAR; LEU5; RFP2; DLEU5; RNF77
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM13
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM13
• Protein Family: Tripartite motif containing
• UniProt Gene Name: TRIM13
• UniProt Synonym Gene Names: LEU5; RFP2; RNF77
• UniProt Entry Name: TRI13_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM13: E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Tumor suppressor; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: endoplasmic reticulum membrane; cytoplasm; integral to membrane

Molecular Function: signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; negative regulation of viral transcription; protein autoubiquitination; ER-associated protein catabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; innate immune response; response to gamma radiation; signal transduction; positive regulation of macroautophagy; activation of NF-kappaB transcription factor