TONSL sirna

TONSL siRNA (Human)

Cat. Num. AAA8201006

• Gene Names: TONSL; IKBR; NFKBIL2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TONSL; TONSL siRNA (Human); IKBR; NFKBIL2; Tonsoku-like protein; Inhibitor of kappa B-related protein; I-kappa-B-related protein; IkappaBR; NF-kappa-B inhibitor-like protein 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2; TONSL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TONSL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,378

• Applicable Applications for TONSL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TONSL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TONSL sirna

siRNA to inhibit TONSL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 187608777
• NCBI GeneID: 4796
• NCBI Accession #: NP_038460.4
• NCBI GenBank Nucleotide #: NM_013432.4
• UniProt Accession #: Q96HA7; Q13006; Q9UGJ2; B5MDP0; C9JKB1; C9JNV8
• Molecular Weight: 150,894 Da
• NCBI Official Full Name: tonsoku-like protein
• NCBI Official Synonym Full Names: tonsoku-like, DNA repair protein
• NCBI Official Symbol: TONSL
• NCBI Official Synonym Symbols: IKBR; NFKBIL2
• NCBI Protein Information: tonsoku-like protein
• UniProt Protein Name: Tonsoku-like protein
• UniProt Gene Name: TONSL
• UniProt Synonym Gene Names: IKBR; NFKBIL2; I-kappa-B-related protein; IkappaBR
• UniProt Entry Name: TONSL_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

NFKBIL2: Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork- associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. Belongs to the Tonsoku family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; DNA replication factor A complex; MCM complex; nuclear replication fork; cytoplasm

Molecular Function: protein binding; histone binding; transcription corepressor activity

Biological Process: cytoplasmic sequestering of transcription factor; double-strand break repair via homologous recombination; replication fork processing