TNKS2 sirna

TNKS2 siRNA (Human)

Cat. Num. AAA8201512

• Gene Names: TNKS2; TNKL; ARTD6; TANK2; pART6; PARP5B; PARP5C; PARP-5b; PARP-5c
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNKS2; TNKS2 siRNA (Human); PARP5B; TANK2; TNKL; Tankyrase-2; ADP-ribosyltransferase diphtheria toxin-like 6; ARTD6; Poly [ADP-ribose] polymerase 5B; TNKS-2; TRF1-interacting ankyrin-related ADP-ribose polymerase 2; Tankyrase II; Tankyrase-like protein; Tankyrase-related protein; TNKS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TNKS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1166

• Applicable Applications for TNKS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TNKS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNKS2 sirna

siRNA to inhibit TNKS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13376842
• NCBI GeneID: 80351
• NCBI Accession #: NP_079511.1
• NCBI GenBank Nucleotide #: NM_025235.3
• UniProt Accession #: Q9H2K2; Q9H8F2; Q9HAS4; B2RBD3
• Molecular Weight: 126,918 Da
• NCBI Official Full Name: tankyrase-2
• NCBI Official Synonym Full Names: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
• NCBI Official Symbol: TNKS2
• NCBI Official Synonym Symbols: TNKL; ARTD6; TANK2; pART6; PARP5B; PARP5C; PARP-5b; PARP-5c
• NCBI Protein Information: tankyrase-2
• UniProt Protein Name: Tankyrase-2
• Protein Family: Tankyrase
• UniProt Gene Name: TNKS2
• UniProt Synonym Gene Names: PARP5B; TANK2; TNKL; TANK2; ARTD6
• UniProt Entry Name: TNKS2_HUMAN

Uniprot Description

TNKS2: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP- ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles.

Protein type: EC 2.4.2.30; Nuclear envelope; Transferase

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: Golgi membrane; pericentriolar material; nuclear chromosome, telomeric region; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; metal ion binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; protein polyubiquitination; Wnt receptor signaling pathway; multicellular organism growth; regulation of multicellular organism growth; positive regulation of telomere maintenance via telomerase