TMEM55B sirna

TMEM55B siRNA (Human)

Cat. Num. AAA8216725

• Gene Names: TMEM55B; C14orf9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEM55B; TMEM55B siRNA (Human); C14orf9; Type 1 phosphatidylinositol 4.5-bisphosphate 4-phosphatase; Type 1 PtdIns-4.5-P2 4-Ptase; PtdIns-4.5-P2 4-Ptase I; Transmembrane protein 55B; TMEM55B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TMEM55B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 149

• Applicable Applications for TMEM55B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMEM55B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEM55B sirna

siRNA to inhibit TMEM55B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 154816186
• NCBI GeneID: 90809
• NCBI Accession #: NP_001094284.1
• NCBI GenBank Nucleotide #: NM_001100814.2
• UniProt Accession #: Q86T03; Q86U09; Q8WUC0; Q9BU67; Q9NSU8; B2RA35
• Molecular Weight: 15,191 Da
• NCBI Official Full Name: type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase isoform 1
• NCBI Official Synonym Full Names: transmembrane protein 55B
• NCBI Official Symbol: TMEM55B
• NCBI Official Synonym Symbols: C14orf9
• NCBI Protein Information: type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
• UniProt Protein Name: Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
• Protein Family: Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
• UniProt Gene Name: TMEM55B
• UniProt Synonym Gene Names: C14orf9; Type 1 PtdIns-4,5-P2 4-Ptase
• UniProt Entry Name: TM55B_HUMAN

NCBI Description

TMEM55B catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate (Ungewickell et al., 2005 [PubMed 16365287]).[supplied by OMIM, Mar 2008]

Uniprot Description

TMEM55B: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 4,5-bisphosphate. Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4- bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3- monophosphate. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.78; Membrane protein, multi-pass; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: late endosome membrane; lysosomal membrane; integral to membrane

Molecular Function: hydrolase activity

Biological Process: metabolic process