TMC8 sirna

TMC8 siRNA (Human)

Cat. Num. AAA8239001

• Gene Names: TMC8; EV2; EVER2; EVIN2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMC8; TMC8 siRNA (Human); EVER2; EVIN2; Transmembrane channel-like protein 8; Epidermodysplasia verruciformis protein 2; TMC8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TMC8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 726

• Applicable Applications for TMC8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMC8 sirna

siRNA to inhibit TMC8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23943900
• NCBI GeneID: 147138
• NCBI Accession #: NP_689681.2
• NCBI GenBank Nucleotide #: NM_152468.4
• UniProt Accession #: Q8IU68; Q2YDC0; Q8IWU7; Q8N358; Q8NF04
• Molecular Weight: 56,132 Da
• NCBI Official Full Name: transmembrane channel-like protein 8
• NCBI Official Synonym Full Names: transmembrane channel-like 8
• NCBI Official Symbol: TMC8
• NCBI Official Synonym Symbols: EV2; EVER2; EVIN2
• NCBI Protein Information: transmembrane channel-like protein 8
• UniProt Protein Name: Transmembrane channel-like protein 8
• Protein Family: Transmembrane channel-like protein
• UniProt Gene Name: TMC8
• UniProt Synonym Gene Names: EVER2; EVIN2
• UniProt Entry Name: TMC8_HUMAN

NCBI Description

Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 8 predicted transmembrane domains and 3 leucine zipper motifs. [provided by RefSeq, Jul 2008]

Uniprot Description

TMC8: an endoplasmic reticulum multipass membrane protein. Defects in TMC8 cause Lutz-Lewandowsky epidermodysplasia verruciformis [MIM:226400], a rare skin disease characterized by increased susceptibility to infection by papillomaviruses with an accompanying high risk of skin carcinoma. Three splice-variant isoforms of the human protein have been described.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: negative regulation of protein oligomerization; zinc ion homeostasis; ion transport; regulation of cell growth; negative regulation of protein binding

Disease: Epidermodysplasia Verruciformis