TET1 sirna

TET1 siRNA (Human)

Cat. Num. AAA8207378

• Gene Names: TET1; LCX; CXXC6; MLL-TET1; TET1-MLL; bA119F7.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TET1; TET1 siRNA (Human); CXXC6; KIAA1676; LCX; Methylcytosine dioxygenase TET1; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Ten-eleven translocation 1 gene protein; TET1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TET1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,136

• Applicable Applications for TET1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TET1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TET1 sirna

siRNA to inhibit TET1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156139122
• NCBI GeneID: 80312
• NCBI Accession #: NP_085128.2
• NCBI GenBank Nucleotide #: NM_030625.2
• UniProt Accession #: Q8NFU7; Q5VUP7; Q7Z6B6; Q8TCR1; Q9C0I7
• Molecular Weight: 235,309 Da
• NCBI Official Full Name: methylcytosine dioxygenase TET1
• NCBI Official Synonym Full Names: tet methylcytosine dioxygenase 1
• NCBI Official Symbol: TET1
• NCBI Official Synonym Symbols: LCX; CXXC6; MLL-TET1; TET1-MLL; bA119F7.1
• NCBI Protein Information: methylcytosine dioxygenase TET1
• UniProt Protein Name: Methylcytosine dioxygenase TET1
• Protein Family: Methylcytosine dioxygenase
• UniProt Gene Name: TET1
• UniProt Synonym Gene Names: CXXC6; KIAA1676; LCX
• UniProt Entry Name: TET1_HUMAN

Uniprot Description

TET1: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC). Might initiate a process leading to cytosine demethylation through deamination into 5- hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'. Belongs to the TET family.

Protein type: Oxidoreductase; DNA-binding; EC 1.14.11.n2

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: nucleus

Molecular Function: structure-specific DNA binding; zinc ion binding; iron ion binding

Biological Process: protein amino acid O-linked glycosylation; inner cell mass cell differentiation; transcription, DNA-dependent; stem cell maintenance; gene expression; positive regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic