TERF2 sirna

TERF2 siRNA (Human)

Cat. Num. AAA8230868

• Gene Names: TERF2; TRF2; TRBF2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TERF2; TERF2 siRNA (Human); TRBF2; TRF2; Telomeric repeat-binding factor 2; TTAGGG repeat-binding factor 2; Telomeric DNA-binding protein; TERF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TERF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 293

• Applicable Applications for TERF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TERF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TERF2 sirna

siRNA to inhibit TERF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 429535832
• NCBI GeneID: 7014
• NCBI Accession #: NP_005643.2
• NCBI GenBank Nucleotide #: NM_005652.4
• UniProt Accession #: Q15554
• Molecular Weight: 32,298 Da
• NCBI Official Full Name: telomeric repeat-binding factor 2
• NCBI Official Synonym Full Names: telomeric repeat binding factor 2
• NCBI Official Symbol: TERF2
• NCBI Official Synonym Symbols: TRF2; TRBF2
• NCBI Protein Information: telomeric repeat-binding factor 2
• UniProt Protein Name: Telomeric repeat-binding factor 2
• Protein Family: Telomeric repeat-binding factor
• UniProt Gene Name: TERF2
• UniProt Synonym Gene Names: TRBF2; TRF2
• UniProt Entry Name: TERF2_HUMAN

NCBI Description

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF2: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: male germ cell nucleus; nucleoplasm; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding

Biological Process: negative regulation of telomere maintenance; telomere capping; in utero embryonic development; negative regulation of telomere maintenance via semi-conservative replication; telomeric loop formation; positive regulation of nitric-oxide synthase activity; positive regulation of telomere maintenance; telomere maintenance via telomerase; cell cycle; age-dependent telomere shortening; telomere maintenance; protection from non-homologous end joining at telomere