TBPL1 sirna

TBPL1 siRNA (Human)

Cat. Num. AAA8237828

• Gene Names: TBPL1; TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBPL1; TBPL1 siRNA (Human); TLF; TLP; TLP21; TRF2; TRP; TATA box-binding protein-like protein 1; TBP-like protein 1; 21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBP-related factor 2; TBP-like factor; TBP-related protein; TBPL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TBPL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 186

• Applicable Applications for TBPL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TBPL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBPL1 sirna

siRNA to inhibit TBPL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 358439409
• NCBI GeneID: 9519
• NCBI Accession #: NP_001240605.1
• NCBI GenBank Nucleotide #: NM_001253676.1
• UniProt Accession #: P62380; O95753; Q9BWD5; Q9Z2Z0; A8K8F5
• Molecular Weight: 20,887 Da
• NCBI Official Full Name: TATA box-binding protein-like protein 1
• NCBI Official Synonym Full Names: TBP-like 1
• NCBI Official Symbol: TBPL1
• NCBI Official Synonym Symbols: TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• NCBI Protein Information: TATA box-binding protein-like protein 1
• UniProt Protein Name: TATA box-binding protein-like protein 1
• Protein Family: TATA box-binding protein-like protein
• UniProt Gene Name: TBPL1
• UniProt Synonym Gene Names: TLF; TLP; TLP21; TRF2; TRP; TBP-like protein 1; STUD; TBP-related factor 2
• UniProt Entry Name: TBPL1_HUMAN

NCBI Description

This gene encodes a member of the TATA box-binding protein family. TATA box-binding proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA box and initiates transcription from TATA-less promoters. This gene plays a critical role in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Nov 2011]

Uniprot Description

TBPL1: Does not bind the TATA box. Has DNA-binding ability. Belongs to the TBP family.

Protein type: Transcription, coactivator/corepressor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q23.2

Cellular Component: cytoplasm; transcription factor TFIIA complex

Molecular Function: DNA binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; acrosome formation; regulation of transcription, DNA-dependent; transcription initiation; spermatid nuclear differentiation; dTTP biosynthetic process