TAF12 sirna

TAF12 siRNA (Human)

Cat. Num. AAA8209676

• Gene Names: TAF12; TAF2J; TAFII20
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAF12; TAF12 siRNA (Human); TAF15; TAF2J; TAFII20; Transcription initiation factor TFIID subunit 12; Transcription initiation factor TFIID 20/15 kDa subunits; TAFII-20/TAFII-15; TAFII20/TAFII15; TAF12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TAF12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for TAF12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAF12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAF12 sirna

siRNA to inhibit TAF12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 206725450
• NCBI GeneID: 6883
• NCBI Accession #: NP_001128690.1
• NCBI GenBank Nucleotide #: NM_001135218.1
• UniProt Accession #: Q16514; Q15775; Q5T077; D3DPM5
• Molecular Weight: 14,825 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 12
• NCBI Official Synonym Full Names: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
• NCBI Official Symbol: TAF12
• NCBI Official Synonym Symbols: TAF2J; TAFII20
• NCBI Protein Information: transcription initiation factor TFIID subunit 12
• UniProt Protein Name: Transcription initiation factor TFIID subunit 12
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF12
• UniProt Synonym Gene Names: TAF15; TAF2J; TAFII20; TAFII-20/TAFII-15; TAFII20/TAFII15
• UniProt Entry Name: TAF12_HUMAN

NCBI Description

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TAF12: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. Belongs to the TAF12 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; RNA elongation from RNA polymerase II promoter; transcription initiation; positive regulation of transcription factor activity; gene expression