SPANXA2 sirna

SPANXA2 siRNA (Human)

Cat. Num. AAA8235420

• Gene Names: SPANXA1; NAP-X; SPANX; CT11.1; SPAN-X; SPAN-Xa; SPAN-Xb; SPANX-A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPANXA2; SPANXA2 siRNA (Human); SPANXA; Sperm protein associated with the nucleus on the X chromosome A; Cancer/testis antigen 11.1; CT11.1; Nuclear-associated protein SPAN-Xa; SPAN-X; SPANX-A; SPANX family member A; SPANXA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPANXA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 97

• Applicable Applications for SPANXA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPANXA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPANXA2 sirna

siRNA to inhibit SPANXA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14192937
• NCBI GeneID: 30014
• NCBI Accession #: NP_038481.2
• NCBI GenBank Nucleotide #: NM_013453.3
• UniProt Accession #: Q9NS26; Q5JWI0
• Molecular Weight: 11,038 Da
• NCBI Official Full Name: sperm protein associated with the nucleus on the X chromosome A
• NCBI Official Synonym Full Names: sperm protein associated with the nucleus, X-linked, family member A1
• NCBI Official Symbol: SPANXA1
• NCBI Official Synonym Symbols: NAP-X; SPANX; CT11.1; SPAN-X; SPAN-Xa; SPAN-Xb; SPANX-A
• NCBI Protein Information: sperm protein associated with the nucleus on the X chromosome A
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome A
• UniProt Gene Name: SPANXA1
• UniProt Synonym Gene Names: SPAN-X; SPANX-A
• UniProt Entry Name: SPNXA_HUMAN

NCBI Description

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-head orientation with SPANX family member A2, which appears to be a duplication of the A1 locus. The protein encoded by this gene targets to the nucleus where it associates with nuclear vacuoles and the redundant nuclear envelope. Based on its association with these poorly characterized regions of the sperm nucleus, this protein provides a biochemical marker to study unique structures in spermatazoa while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

SPANXA1: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-head orientation with SPANX family member A2, which appears to be a duplication of the A1 locus. The protein encoded by this gene targets to the nucleus where it associates with nuclear vacuoles and the redundant nuclear envelope. Based on its association with these poorly characterized regions of the sperm nucleus, this protein provides a biochemical marker to study unique structures in spermatazoa while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus

Biological Process: spermatogenesis