SP100 sirna

SP100 siRNA (Human)

Cat. Num. AAA8234124

• Gene Names: SP100; lysp100b
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SP100; SP100 siRNA (Human); Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa; SP100 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SP100 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 885

• Applicable Applications for SP100 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SP100 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SP100 sirna

siRNA to inhibit SP100 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 122939208
• NCBI GeneID: 6672
• NCBI Accession #: NP_001073860.1
• NCBI GenBank Nucleotide #: NM_001080391.1
• UniProt Accession #: P23497; O75450; Q13343; Q8TE34; Q96F70; Q96T24; Q96T95; B4DDX5; B8ZZD8; E7EUA7; E9PH61; F8WFE2
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: nuclear autoantigen Sp-100 isoform 1
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100
• UniProt Entry Name: SP100_HUMAN

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

SP100: May play a role in the control of gene expression. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; Mre11 complex; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein homodimerization activity; DNA binding; transcription coactivator activity; chromatin binding; transcription corepressor activity; transcription factor binding; kinase binding

Biological Process: retinoic acid receptor signaling pathway; response to retinoic acid; negative regulation of protein export from nucleus; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of transcription factor activity; negative regulation of cell motility; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; chromatin remodeling; negative regulation of viral transcription; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; response to cytokine stimulus; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; telomere maintenance