SORCS3 sirna

SORCS3 siRNA (Human)

Cat. Num. AAA8241204

• Gene Names: SORCS3; SORCS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SORCS3; SORCS3 siRNA (Human); KIAA1059; VPS10 domain-containing receptor SorCS3; SORCS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SORCS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,222

• Applicable Applications for SORCS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SORCS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SORCS3 sirna

siRNA to inhibit SORCS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18379346
• NCBI GeneID: 22986
• NCBI Accession #: NP_055793.1
• NCBI GenBank Nucleotide #: NM_014978.2
• UniProt Accession #: Q9UPU3; Q5VXF9; Q9NQJ2
• Molecular Weight: 135,755 Da
• NCBI Official Full Name: VPS10 domain-containing receptor SorCS3
• NCBI Official Synonym Full Names: sortilin-related VPS10 domain containing receptor 3
• NCBI Official Symbol: SORCS3
• NCBI Official Synonym Symbols: SORCS
• NCBI Protein Information: VPS10 domain-containing receptor SorCS3
• UniProt Protein Name: VPS10 domain-containing receptor SorCS3
• Protein Family: VPS10 domain-containing receptor
• UniProt Gene Name: SORCS3
• UniProt Synonym Gene Names: KIAA1059
• UniProt Entry Name: SORC3_HUMAN

NCBI Description

This gene encodes a type-I receptor transmembrane protein that is a member of the vacuolar protein sorting 10 receptor family. Proteins of this family are defined by a vacuolar protein sorting 10 domain at the N-terminus. The N-terminal segment of this domain has a consensus motif for proprotein convertase processing, and the C-terminal segment of this domain is characterized by ten conserved cysteine residues. The vacuolar protein sorting 10 domain is followed by a leucine-rich segment, a transmembrane domain, and a short C-terminal cytoplasmic domain that interacts with adaptor molecules. The transcript is expressed at high levels in the brain, and candidate gene studies suggest that genetic variation in this gene is associated with Alzheimer's disease. Consistent with this observation, knockdown of the gene in cell culture results in an increase in amyloid precursor protein processing. [provided by RefSeq, Dec 2014]

Uniprot Description

SORCS3: one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: membrane; integral to membrane

Molecular Function: neuropeptide receptor activity

Biological Process: neuropeptide signaling pathway; learning; memory