SORBS1 sirna

SORBS1 siRNA (Human)

Cat. Num. AAA8235463

• Gene Names: SORBS1; CAP; FLAF2; R85FL; SH3D5; SORB1; SH3P12
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SORBS1; SORBS1 siRNA (Human); KIAA0894; KIAA1296; SH3D5; Sorbin and SH3 domain-containing protein 1; Ponsin; SH3 domain protein 5; SH3P12; c-Cbl-associated protein; CAP; SORBS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SORBS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1292

• Applicable Applications for SORBS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SORBS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SORBS1 sirna

siRNA to inhibit SORBS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 78000163
• NCBI GeneID: 10580
• NCBI Accession #: NP_001030126.1
• NCBI GenBank Nucleotide #: NM_001034954.1
• UniProt Accession #: Q9BX66; O43857; Q5T923; Q5T924; Q5T927; Q5T928; A0AED4; A6NEK3; A6NID8; A6NJS4; A7MD40; D3DR42
• Molecular Weight: 143,741 Da
• NCBI Official Full Name: sorbin and SH3 domain-containing protein 1 isoform 3
• NCBI Official Synonym Full Names: sorbin and SH3 domain containing 1
• NCBI Official Symbol: SORBS1
• NCBI Official Synonym Symbols: CAP; FLAF2; R85FL; SH3D5; SORB1; SH3P12
• NCBI Protein Information: sorbin and SH3 domain-containing protein 1
• UniProt Protein Name: Sorbin and SH3 domain-containing protein 1
• Protein Family: Sorbin and SH3 domain-containing protein
• UniProt Gene Name: SORBS1
• UniProt Synonym Gene Names: CAP
• UniProt Entry Name: SRBS1_HUMAN

NCBI Description

This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

SORBS1: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin- stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: centrosome; focal adhesion; nuclear matrix; zonula adherens; cytosol; cell-substrate adherens junction; lipid raft; nucleoplasm; cell-cell adherens junction; cytoplasm; plasma membrane; stress fiber; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; cytoskeletal protein binding; actin binding; protein kinase binding; insulin receptor binding

Biological Process: focal adhesion formation; positive regulation of glucose import; cellular response to insulin stimulus; muscle contraction; positive regulation of signal transduction; cell-matrix adhesion; positive regulation of glycogen biosynthetic process; stress fiber formation; insulin receptor signaling pathway; positive regulation of lipid biosynthetic process; glucose transport