SNRNP35 sirna

SNRNP35 siRNA (Human)

Cat. Num. AAA8209434

• Gene Names: SNRNP35; HM-1; U1SNRNPBP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNRNP35; SNRNP35 siRNA (Human); HM1; U1SNRNPBP; U11/U12 small nuclear ribonucleoprotein 35 kDa protein; U11/U12 snRNP 35 kDa protein; U11/U12-35K; Protein HM-1; U1 snRNP-binding protein homolog; SNRNP35 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNRNP35 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 246

• Applicable Applications for SNRNP35 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNRNP35 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNRNP35 sirna

siRNA to inhibit SNRNP35 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13027642
• NCBI GeneID: 11066
• NCBI Accession #: NP_073208.1
• NCBI GenBank Nucleotide #: NM_022717.3
• UniProt Accession #: Q16560; Q5XKN9; A8K262
• Molecular Weight: 29,992 Da
• NCBI Official Full Name: U11/U12 small nuclear ribonucleoprotein 35 kDa protein isoform a
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein 35kDa (U11/U12)
• NCBI Official Symbol: SNRNP35
• NCBI Official Synonym Symbols: HM-1; U1SNRNPBP
• NCBI Protein Information: U11/U12 small nuclear ribonucleoprotein 35 kDa protein
• UniProt Protein Name: U11/U12 small nuclear ribonucleoprotein 35 kDa protein
• Protein Family: U11/U12 small nuclear ribonucleoprotein 35 kDa protein
• UniProt Gene Name: SNRNP35
• UniProt Synonym Gene Names: HM1; U1SNRNPBP; U11/U12 snRNP 35 kDa protein; U11/U12-35K
• UniProt Entry Name: U1SBP_HUMAN

NCBI Description

The protein encoded by this gene is a homolog of the U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, which is a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. Alternative splicing results in multiple transcript variants encoding two distinct isoforms and representing a non-protein coding variant. [provided by RefSeq, Aug 2013]

Uniprot Description

U1SNRNPBP: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleus; U12-dependent spliceosome

Molecular Function: RNA binding; nucleotide binding

Biological Process: RNA splicing; mRNA processing