SNAPC4 sirna

SNAPC4 siRNA (Human)

Cat. Num. AAA8240126

• Gene Names: SNAPC4; SNAP190; PTFalpha
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNAPC4; SNAPC4 siRNA (Human); SNAP190; snRNA-activating protein complex subunit 4; SNAPc subunit 4; Proximal sequence element-binding transcription factor subunit alpha; PSE-binding factor subunit alpha; PTF subunit alpha; snRNA-activating protein complex 190 kDa subunit; SNAPc 190 kDa subunit; SNAPC4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNAPC4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1469

• Applicable Applications for SNAPC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNAPC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNAPC4 sirna

siRNA to inhibit SNAPC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 92859678
• NCBI GeneID: 6621
• NCBI Accession #: NP_003077.2
• NCBI GenBank Nucleotide #: NM_003086.2
• UniProt Accession #: Q5SXM2; Q9Y6P7
• Molecular Weight: 159,433 Da
• NCBI Official Full Name: snRNA-activating protein complex subunit 4
• NCBI Official Synonym Full Names: small nuclear RNA activating complex, polypeptide 4, 190kDa
• NCBI Official Symbol: SNAPC4
• NCBI Official Synonym Symbols: SNAP190; PTFalpha
• NCBI Protein Information: snRNA-activating protein complex subunit 4
• UniProt Protein Name: snRNA-activating protein complex subunit 4
• Protein Family: snRNA-activating protein complex
• UniProt Gene Name: SNAPC4
• UniProt Synonym Gene Names: SNAPc subunit 4; PSE-binding factor subunit alpha; PTF subunit alpha; SNAPc 190 kDa subunit
• UniProt Entry Name: SNPC4_HUMAN

Uniprot Description

SNAP190: Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; snRNA-activating protein complex; nucleus

Molecular Function: DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription from RNA polymerase III promoter; regulation of transcription, DNA-dependent; snRNA transcription; snRNA transcription from RNA polymerase II promoter; gene expression; snRNA transcription from RNA polymerase III promoter