SLX4 sirna

SLX4 siRNA (Human)

Cat. Num. AAA8212037

• Gene Names: SLX4; FANCP; BTBD12; MUS312
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLX4; SLX4 siRNA (Human); BTBD12; KIAA1784; KIAA1987; Structure-specific endonuclease subunit SLX4; BTB/POZ domain-containing protein 12; SLX4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLX4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1834

• Applicable Applications for SLX4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLX4 sirna

siRNA to inhibit SLX4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 63252863
• NCBI GeneID: 84464
• NCBI Accession #: NP_115820.2
• NCBI GenBank Nucleotide #: NM_032444.2
• UniProt Accession #: Q8IY92; Q69YT8; Q8TF15; Q96JP1
• Molecular Weight: 165,903 Da
• NCBI Official Full Name: structure-specific endonuclease subunit SLX4
• NCBI Official Synonym Full Names: SLX4 structure-specific endonuclease subunit
• NCBI Official Symbol: SLX4
• NCBI Official Synonym Symbols: FANCP; BTBD12; MUS312
• NCBI Protein Information: structure-specific endonuclease subunit SLX4
• UniProt Protein Name: Structure-specific endonuclease subunit SLX4
• Protein Family: Structure-specific endonuclease
• UniProt Gene Name: SLX4
• UniProt Synonym Gene Names: BTBD12; KIAA1784; KIAA1987
• UniProt Entry Name: SLX4_HUMAN

NCBI Description

This gene encodes a structure-specific endonuclease subunit. The encoded protein contains a central BTB domain and it forms a multiprotein complex with the ERCC4(XPF)-ERCC1, MUS81-EME1, and SLX1 endonucleases, and also associates with MSH2/MSH3 mismatch repair complex, telomere binding complex TERF2(TRF2)-TERF2IP(RAP1), the protein kinase PLK1 and the uncharacterized protein C20orf94. The multiprotein complex is required for repair of specific types of DNA lesions and is critical for cellular responses to replication fork failure. The encoded protein acts as a docking platform for the assembly of multiple structure-specific endonucleases.[provided by RefSeq, Jan 2011]

Uniprot Description

SLX4: regulatory subunit of different structure-specific endonucleases. Required for DNA recombination and repair. Together with SLX1 forms a Holliday junction (HJ) resolvase. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating HJs. Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. Required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. Involved in Rad1/Rad10pdependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and C20ORF94. Localizes to sites of DNA damage. Two alternatively spliced human isoforms have been described.

Protein type: Deoxyribonuclease; DNA repair, damage

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; nuclear chromosome, telomeric region; nuclear chromatin; cytoplasm; cell junction

Molecular Function: protein binding; 5'-flap endonuclease activity; 3'-flap endonuclease activity; enzyme activator activity; crossover junction endodeoxyribonuclease activity

Biological Process: positive regulation of catalytic activity; nucleotide-excision repair; DNA replication; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination

Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group P