SLC7A14 sirna

SLC7A14 siRNA (Human)

Cat. Num. AAA8233162

• Gene Names: SLC7A14; PPP1R142
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC7A14; SLC7A14 siRNA (Human); KIAA1613; Probable cationic amino acid transporter; Solute carrier family 7 member 14; SLC7A14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC7A14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 771

• Applicable Applications for SLC7A14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC7A14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC7A14 sirna

siRNA to inhibit SLC7A14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 181337167
• NCBI GeneID: 57709
• NCBI Accession #: NP_066000.2
• NCBI GenBank Nucleotide #: NM_020949.2
• UniProt Accession #: Q8TBB6; Q9HCF9; B3KV33
• Molecular Weight: 84,052 Da
• NCBI Official Full Name: probable cationic amino acid transporter
• NCBI Official Synonym Full Names: solute carrier family 7, member 14
• NCBI Official Symbol: SLC7A14
• NCBI Official Synonym Symbols: PPP1R142
• NCBI Protein Information: probable cationic amino acid transporter
• UniProt Protein Name: Probable cationic amino acid transporter
• Protein Family: Probable cationic amino acid transporter
• UniProt Gene Name: SLC7A14
• UniProt Synonym Gene Names: KIAA1613
• UniProt Entry Name: S7A14_HUMAN

NCBI Description

This gene is predicted to encode a glycosylated, cationic amino acid transporter protein with 14 transmembrane domains. This gene is primarily expressed in skin fibroblasts, neural tissue, and primary endothelial cells and its protein is predicted to mediate lysosomal uptake of cationic amino acids. Mutations in this gene are associated with autosomal recessive retinitis pigmentosa. In mice, this gene is expressed in the photoreceptor layer of the retina where its expression increases over the course of retinal development and persists in the mature retina. [provided by RefSeq, Apr 2014]

Uniprot Description

SLC7A14: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: lysosomal membrane; integral to membrane

Molecular Function: amino acid transmembrane transporter activity

Disease: Retinitis Pigmentosa 68