SLC52A1 sirna

SLC52A1 siRNA (Human)

Cat. Num. AAA8240786

• Gene Names: SLC52A1; PAR2; RFT1; RBFVD; RFVT1; hRFT1; GPCR42; GPR172B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC52A1; SLC52A1 siRNA (Human); GPR172B; PAR2; RFT1; Solute carrier family 52. riboflavin transporter. member 1; Porcine endogenous retrovirus A receptor 2; PERV-A receptor 2; Protein GPR172B; Riboflavin transporter 1; hRFT1; SLC52A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC52A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 448

• Applicable Applications for SLC52A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC52A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC52A1 sirna

siRNA to inhibit SLC52A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157388951
• NCBI GeneID: 55065
• NCBI Accession #: NP_001098047.1
• NCBI GenBank Nucleotide #: NM_001104577.1
• UniProt Accession #: Q9NWF4; Q6P9E0; Q86UT0; B5MEV1; B5MEV2
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: solute carrier family 52, riboflavin transporter, member 1
• NCBI Official Synonym Full Names: solute carrier family 52 (riboflavin transporter), member 1
• NCBI Official Symbol: SLC52A1
• NCBI Official Synonym Symbols: PAR2; RFT1; RBFVD; RFVT1; hRFT1; GPCR42; GPR172B
• NCBI Protein Information: solute carrier family 52, riboflavin transporter, member 1
• UniProt Protein Name: Solute carrier family 52, riboflavin transporter, member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC52A1
• UniProt Synonym Gene Names: GPR172B; PAR2; RFT1; PERV-A receptor 2; hRFT1
• UniProt Entry Name: S52A1_HUMAN

NCBI Description

Biological redox reactions require electron donors and acceptor. Vitamin B2 is the source for the flavin in flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) which are common redox reagents. This gene encodes a member of the riboflavin (vitamin B2) transporter family. Haploinsufficiency of this protein can cause maternal riboflavin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013]

Uniprot Description

GPR172B: Riboflavin transporter. Riboflavin transport is Na(+)- independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin. Weakly inhibited by flavin adenine dinucleotide (FAD). In case of infection by retroviruses, acts as a cell receptor to retroviral envelopes similar to the porcine endogenous retrovirus (PERV-A). Haploinsufficiency in SLC52A1 can cause maternal riboflavin deficiency. In the newborn infant, this can lead to a transient riboflavin-responsive disorder with clinical and biochemical features of multiple acyl-CoA dehydrogenase deficiency. Belongs to the riboflavin transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: integral to plasma membrane

Molecular Function: viral receptor activity; riboflavin transporter activity

Biological Process: entry of virus into host cell; riboflavin transport

Disease: Riboflavin Deficiency