SLC35B3 sirna

SLC35B3 siRNA (Human)

Cat. Num. AAA8226903

• Gene Names: SLC35B3; CGI-19; PAPST2; C6orf196
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC35B3; SLC35B3 siRNA (Human); C6orf196; PAPST2; Adenosine 3'-phospho 5'-phosphosulfate transporter 2; 3'-phosphoadenosine 5'-phosphosulfate transporter; PAPS transporter 2; Solute carrier family 35 member B3; SLC35B3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC35B3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 283

• Applicable Applications for SLC35B3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC35B3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC35B3 sirna

siRNA to inhibit SLC35B3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 216547942
• NCBI GeneID: 51000
• NCBI Accession #: NP_001136013.1
• NCBI GenBank Nucleotide #: NM_001142541.2
• UniProt Accession #: Q9H1N7; Q1XH11; Q6MZJ0; Q7Z662; Q9Y308; A6NKX9
• Molecular Weight: 31,531 Da
• NCBI Official Full Name: adenosine 3'-phospho 5'-phosphosulfate transporter 2
• NCBI Official Synonym Full Names: solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B3
• NCBI Official Symbol: SLC35B3
• NCBI Official Synonym Symbols: CGI-19; PAPST2; C6orf196
• NCBI Protein Information: adenosine 3'-phospho 5'-phosphosulfate transporter 2
• UniProt Protein Name: Adenosine 3'-phospho 5'-phosphosulfate transporter 2
• Protein Family: Adenosine 3'-phospho 5'-phosphosulfate transporter
• UniProt Gene Name: SLC35B3
• UniProt Synonym Gene Names: C6orf196; PAPST2
• UniProt Entry Name: S35B3_HUMAN

NCBI Description

This gene is a member of the solute carrier family. The encoded protein is involved in the transport of 3-prime phosphoadenosine 5-prime phosphosulfate (PAPS) from the nucleus or the cytosol to the Golgi lumen. This gene has been reported to be expressed preferentially in the human colon tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

SLC35B3: Mediates the transport of adenosine 3'-phospho 5'- phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon. Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: Golgi membrane; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; integral to membrane

Biological Process: glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; transmembrane transport; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process