SLC22A16 sirna

SLC22A16 siRNA (Human)

Cat. Num. AAA8216234

• Gene Names: SLC22A16; CT2; OAT6; OCT6; OKB1; FLIPT2; dJ261K5.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC22A16; SLC22A16 siRNA (Human); Solute carrier family 22 member 16; Carnitine transporter 2; CT2; Fly-like putative transporter 2; FLIPT2; Flipt 2; Organic cation transporter OKB1; Organic cation/carnitine transporter 6; SLC22A16 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC22A16 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 515

• Applicable Applications for SLC22A16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC22A16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC22A16 sirna

siRNA to inhibit SLC22A16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542327
• NCBI GeneID: 85413
• NCBI Accession #: NP_149116.2
• NCBI GenBank Nucleotide #: NM_033125.3
• UniProt Accession #: Q86VW1; O14567; Q5JXM1; Q8IUG8; Q8IZD5; Q96M90; Q96RU0
• Molecular Weight: 58,061 Da
• NCBI Official Full Name: solute carrier family 22 member 16
• NCBI Official Synonym Full Names: solute carrier family 22 (organic cation/carnitine transporter), member 16
• NCBI Official Symbol: SLC22A16
• NCBI Official Synonym Symbols: CT2; OAT6; OCT6; OKB1; FLIPT2; dJ261K5.1
• NCBI Protein Information: solute carrier family 22 member 16
• UniProt Protein Name: Solute carrier family 22 member 16
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC22A16
• UniProt Synonym Gene Names: OCT6; CT2; FLIPT2; Flipt 2
• UniProt Entry Name: S22AG_HUMAN

NCBI Description

This gene encodes a member of the organic zwitterion transporter protein family which transports carnitine. The encoded protein has also been shown to transport anticancer drugs like bleomycin (PMID: 20037140) successful treatment has been correlated with the level of activity of this transporter in tumor cells. [provided by RefSeq, Dec 2011]

Uniprot Description

SLC22A16: High affinity carnitine transporter; the uptake is partially sodium-ion dependent. Thought to mediate the L-carnitine secretion mechanism from testis epididymal epithelium into the lumen which is involved in the maturation of spermatozoa. Also transports organic cations such as tetraethylammonium (TEA) and doxorubicin. The uptake of TEA is inhibited by various organic cations. The uptake of doxorubicin is sodium-independent. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Motility/polarity/chemotaxis; Transporter

Chromosomal Location of Human Ortholog: 6q22.1|6q21-q22.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: amine transmembrane transporter activity; carnitine transporter activity; organic cation transmembrane transporter activity

Biological Process: sperm motility; organic cation transport; acid secretion; amine transport; single fertilization; multicellular organismal development; spermatogenesis; cell differentiation; transmembrane transport; carnitine transport