SLC1A5 sirna

SLC1A5 siRNA (Human)

Cat. Num. AAA827427

• Gene Names: SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC1A5; SLC1A5 siRNA (Human); ASCT2; M7V1; RDR; RDRC; Neutral amino acid transporter B(0; ATB(0); Baboon M7 virus receptor; RD114/simian type D retrovirus receptor; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5; SLC1A5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC1A5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 313

• Applicable Applications for SLC1A5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC1A5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC1A5 sirna

siRNA to inhibit SLC1A5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 223468564
• NCBI GeneID: 6510
• NCBI Accession #: NP_001138616.1
• NCBI GenBank Nucleotide #: NM_001145144.1
• UniProt Accession #: Q15758; O95720; Q96RL9; Q9BWQ3; Q9UNP2; A8K9H5; B4DR77; B4DWS4; B7ZB81; D0EYG6; E9PC01
• Molecular Weight: 33,710 Da
• NCBI Official Full Name: neutral amino acid transporter B(0) isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 (neutral amino acid transporter), member 5
• NCBI Official Symbol: SLC1A5
• NCBI Official Synonym Symbols: R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
• NCBI Protein Information: neutral amino acid transporter B(0)
• UniProt Protein Name: Neutral amino acid transporter B(0)
• Protein Family: Neutral amino acid transporter
• UniProt Gene Name: SLC1A5
• UniProt Synonym Gene Names: ASCT2; M7V1; RDR; RDRC; ATB(0)
• UniProt Entry Name: AAAT_HUMAN

NCBI Description

The SLC1A5 gene encodes a sodium-dependent neutral amino acid transporter that can act as a receptor for RD114/type D retrovirus (Larriba et al., 2001 [PubMed 11781704]).[supplied by OMIM, Jan 2011]

Uniprot Description

SLC1A5: Has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. Act as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses. Placenta, lung, skeletal muscle, kidney, pancreas, and intestine. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A5 subfamily. 1 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: Golgi apparatus; membrane; integral to plasma membrane; melanosome; plasma membrane

Molecular Function: viral receptor activity; L-serine transmembrane transporter activity; protein binding; neutral amino acid transmembrane transporter activity; receptor activity; L-glutamine transmembrane transporter activity; sodium:dicarboxylate symporter activity

Biological Process: neutral amino acid transport; extracellular amino acid transport; entry of virus into host cell; amino acid transport; dicarboxylic acid transport; ion transport; glutamine transport; transmembrane transport; L-serine transport