SLBP sirna

SLBP siRNA (Human)

Cat. Num. AAA8201713

• Gene Names: SLBP; HBP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLBP; SLBP siRNA (Human); HBP; Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; SLBP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLBP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 235

• Applicable Applications for SLBP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLBP sirna

siRNA to inhibit SLBP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 807045903
• NCBI GeneID: 7884
• NCBI Accession #: NP_001293003.1
• NCBI GenBank Nucleotide #: NM_001306074.1
• UniProt Accession #: Q14493; B3KRJ5
• Molecular Weight: 26,695 Da
• NCBI Official Full Name: histone RNA hairpin-binding protein isoform 2
• NCBI Official Synonym Full Names: stem-loop binding protein
• NCBI Official Symbol: SLBP
• NCBI Official Synonym Symbols: HBP
• NCBI Protein Information: histone RNA hairpin-binding protein
• UniProt Protein Name: Histone RNA hairpin-binding protein
• UniProt Gene Name: SLBP
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: SLBP_HUMAN

NCBI Description

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008]

Uniprot Description

SLBP: RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs. Monomer. SLBP/pre-mRNA complex interacts with ZNF473. Interacts with the Importin alpha/Importin beta receptor, LSM1, MIF4GD, TNPO3 and UPF1. Interaction with LSM1 occurs when histone mRNA is being rapidly degraded during the S phase. Found in a ternary complex with ERI1 and the stem-loop structure of the 3' end of histone mRNA. Associates with polyribosomes. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Binds in a cooperative manner with ERI1 to the mature 3'-end of histone mRNAs. Widely expressed. Belongs to the SLBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm; ribonucleoprotein complex; cytosol; nucleus

Molecular Function: mRNA binding; protein binding

Biological Process: transcription from RNA polymerase II promoter; mRNA export from nucleus; mRNA transport; histone mRNA metabolic process; DNA replication during S phase; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription