SKIL sirna

SKIL siRNA (Human)

Cat. Num. AAA826904

• Gene Names: SKIL; SNO; SnoA; SnoI; SnoN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SKIL; SKIL siRNA (Human); SNO; Ski-like protein; Ski-related oncogene; Ski-related protein; SKIL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SKIL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 638

• Applicable Applications for SKIL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SKIL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SKIL sirna

siRNA to inhibit SKIL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 223029420
• NCBI GeneID: 6498
• NCBI Accession #: NP_001138569.1
• NCBI GenBank Nucleotide #: NM_001145097.2
• UniProt Accession #: P12757; O00464; P12756; Q07501; A6NGT1; B4DT50
• Molecular Weight: 74,784 Da
• NCBI Official Full Name: ski-like protein isoform 2
• NCBI Official Synonym Full Names: SKI-like proto-oncogene
• NCBI Official Symbol: SKIL
• NCBI Official Synonym Symbols: SNO; SnoA; SnoI; SnoN
• NCBI Protein Information: ski-like protein
• UniProt Protein Name: Ski-like protein
• Protein Family: Ski-like protein
• UniProt Gene Name: SKIL
• UniProt Synonym Gene Names: SNO
• UniProt Entry Name: SKIL_HUMAN

NCBI Description

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; response to antibiotic; regulation of apoptosis; negative regulation of cell differentiation; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; positive regulation of axonogenesis; spermatogenesis; gene expression; cell cycle arrest; negative regulation of transforming growth factor beta receptor signaling pathway; lymphocyte homeostasis