SH3BP4 sirna

SH3BP4 siRNA (Human)

Cat. Num. AAA8207737

• Gene Names: SH3BP4; TTP; BOG25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH3BP4; SH3BP4 siRNA (Human); BOG25; EHB10; TTP; SH3 domain-binding protein 4; EH-binding protein 10; Transferrin receptor-trafficking protein; SH3BP4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SH3BP4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 552

• Applicable Applications for SH3BP4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH3BP4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH3BP4 sirna

siRNA to inhibit SH3BP4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657562
• NCBI GeneID: 23677
• NCBI Accession #: NP_055336.1
• NCBI GenBank Nucleotide #: NM_014521.2
• UniProt Accession #: Q9P0V3; O95082; Q309A3; Q53QD0; Q53TD1
• Molecular Weight: 60,337 Da
• NCBI Official Full Name: SH3 domain-binding protein 4
• NCBI Official Synonym Full Names: SH3-domain binding protein 4
• NCBI Official Symbol: SH3BP4
• NCBI Official Synonym Symbols: TTP; BOG25
• NCBI Protein Information: SH3 domain-binding protein 4
• UniProt Protein Name: SH3 domain-binding protein 4
• Protein Family: SH3 domain-binding protein
• UniProt Gene Name: SH3BP4
• UniProt Synonym Gene Names: BOG25; EHB10; TTP
• UniProt Entry Name: SH3B4_HUMAN

NCBI Description

This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of a specific protein receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

SH3BP4: Functions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin- mediated endocytosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Vesicle

Chromosomal Location of Human Ortholog: 2q37.1-q37.2

Cellular Component: clathrin-coated vesicle; cytoplasm; coated pit; nucleus

Molecular Function: identical protein binding; protein binding; Ras GTPase binding; GDP-dissociation inhibitor activity

Biological Process: negative regulation of cell proliferation; negative regulation of TOR signaling pathway; regulation of catalytic activity; endocytosis; negative regulation of cell growth; positive regulation of autophagy; amino acid import