SGCZ sirna

SGCZ siRNA (Human)

Cat. Num. AAA8211066

• Gene Names: SGCZ; ZSG1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SGCZ; SGCZ siRNA (Human); Zeta-sarcoglycan; Zeta-SG; ZSG1; SGCZ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SGCZ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 312

• Applicable Applications for SGCZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SGCZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SGCZ sirna

siRNA to inhibit SGCZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 94721265
• NCBI GeneID: 137868
• NCBI Accession #: NP_631906.2
• NCBI GenBank Nucleotide #: NM_139167.2
• UniProt Accession #: Q96LD1; Q6REU0
• Molecular Weight: 34,494 Da
• NCBI Official Full Name: zeta-sarcoglycan
• NCBI Official Synonym Full Names: sarcoglycan, zeta
• NCBI Official Symbol: SGCZ
• NCBI Official Synonym Symbols: ZSG1
• NCBI Protein Information: zeta-sarcoglycan
• UniProt Protein Name: Zeta-sarcoglycan
• Protein Family: Zeta-sarcoglycan
• UniProt Gene Name: SGCZ
• UniProt Synonym Gene Names: Zeta-SG
• UniProt Entry Name: SGCZ_HUMAN

NCBI Description

The zeta-sarcoglycan gene measures over 465 kb and localizes to 8p22. This protein is part of the sarcoglycan complex, a group of 6 proteins. The sarcoglycans are all N-glycosylated transmembrane proteins with a short intra-cellular domain, a single transmembrane region and a large extra-cellular domain containing a carboxyl-terminal cluster with several conserved cysteine residues. The sarcoglycan complex is part of the dystrophin-associated glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extra-cellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

SGCZ: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. May play a role in the maintenance of striated muscle membrane stability. Belongs to the sarcoglycan beta/delta/gamma/zeta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytoskeleton; cytoplasm; integral to membrane; sarcoglycan complex; sarcolemma

Biological Process: cardiac muscle development; muscle maintenance; muscle development; heart contraction; muscle cell development