SETDB2 sirna

SETDB2 siRNA (Human)

Cat. Num. AAA8206261

• Gene Names: SETDB2; CLLD8; CLLL8; KMT1F; C13orf4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SETDB2; SETDB2 siRNA (Human); C13orf4; CLLD8; KMT1F; Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SETDB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 718

• Applicable Applications for SETDB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SETDB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SETDB2 sirna

siRNA to inhibit SETDB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 238624099
• NCBI GeneID: 83852
• NCBI Accession #: NP_001153780.1
• NCBI GenBank Nucleotide #: NM_001160308.1
• UniProt Accession #: Q96T68; Q5TC65; Q5TC66; Q5W0A7; Q659A7; Q86UD6; Q96AI6
• Molecular Weight: 81,766 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETDB2 isoform b
• NCBI Official Synonym Full Names: SET domain, bifurcated 2
• NCBI Official Symbol: SETDB2
• NCBI Official Synonym Symbols: CLLD8; CLLL8; KMT1F; C13orf4
• NCBI Protein Information: histone-lysine N-methyltransferase SETDB2
• UniProt Protein Name: Histone-lysine N-methyltransferase SETDB2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETDB2
• UniProt Synonym Gene Names: C13orf4; CLLD8; KMT1F
• UniProt Entry Name: SETB2_HUMAN

NCBI Description

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM, Mar 2008]

Uniprot Description

SETDB2: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: nucleoplasm; chromosome; nucleus

Molecular Function: DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K9 specific)

Biological Process: mitosis; establishment and/or maintenance of chromatin architecture; cell division; heart looping; negative regulation of transcription, DNA-dependent; chromosome segregation; histone H3-K9 methylation