SERPINA3 sirna

SERPINA3 siRNA (Human)

Cat. Num. AAA8231588

• Gene Names: SERPINA3; ACT; AACT; GIG24; GIG25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SERPINA3; SERPINA3 siRNA (Human); AACT; Alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3; SERPINA3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SERPINA3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for SERPINA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SERPINA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SERPINA3 sirna

siRNA to inhibit SERPINA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50659080
• NCBI GeneID: 12
• NCBI Accession #: NP_001076.2
• NCBI GenBank Nucleotide #: NM_001085.4
• UniProt Accession #: P01011; Q13703; Q2TU87; Q2TU88; Q59GP9; Q6LBY8; Q6LDT7; Q6NSC9; Q8N177; Q96DW8; Q9UC47; B3KVQ7
• Molecular Weight: 10,717 Da
• NCBI Official Full Name: alpha-1-antichymotrypsin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• NCBI Official Symbol: SERPINA3
• NCBI Official Synonym Symbols: ACT; AACT; GIG24; GIG25
• NCBI Protein Information: alpha-1-antichymotrypsin
• UniProt Protein Name: Alpha-1-antichymotrypsin
• Protein Family: Alpha-1-antichymotrypsin
• UniProt Gene Name: SERPINA3
• UniProt Synonym Gene Names: AACT; ACT
• UniProt Entry Name: AACT_HUMAN

NCBI Description

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space; extracellular region; intracellular; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; DNA binding

Biological Process: regulation of lipid metabolic process; maintenance of gastrointestinal epithelium; acute-phase response; inflammatory response