RPL22 sirna

RPL22 siRNA (Human)

Cat. Num. AAA8226120

• Gene Names: RPL22; EAP; L22; HBP15; HBP15/L22
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPL22; RPL22 siRNA (Human); 60S ribosomal protein L22; EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15; RPL22 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RPL22 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 128

• Applicable Applications for RPL22 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPL22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPL22 sirna

siRNA to inhibit RPL22 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506613
• NCBI GeneID: 6146
• NCBI Accession #: NP_000974.1
• NCBI GenBank Nucleotide #: NM_000983.3
• UniProt Accession #: P35268; Q6IBD1; B2R495
• Molecular Weight: 14,787 Da
• NCBI Official Full Name: 60S ribosomal protein L22 proprotein
• NCBI Official Synonym Full Names: ribosomal protein L22
• NCBI Official Symbol: RPL22
• NCBI Official Synonym Symbols: EAP; L22; HBP15; HBP15/L22
• NCBI Protein Information: 60S ribosomal protein L22
• UniProt Protein Name: 60S ribosomal protein L22
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL22
• UniProt Synonym Gene Names: EAP
• UniProt Entry Name: RL22_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL22: a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: focal adhesion; cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: heparin binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; alpha-beta T cell differentiation; viral transcription; gene expression; viral infectious cycle; translational termination