ROM1 sirna

ROM1 siRNA (Human)

Cat. Num. AAA8232007

• Gene Names: ROM1; ROM; RP7; ROSP1; TSPAN23
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ROM1; ROM1 siRNA (Human); TSPAN23; Rod outer segment membrane protein 1; ROSP1; Tetraspanin-23; Tspan-23; ROM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ROM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 351

• Applicable Applications for ROM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ROM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ROM1 sirna

siRNA to inhibit ROM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506575
• NCBI GeneID: 6094
• NCBI Accession #: NP_000318.1
• NCBI GenBank Nucleotide #: NM_000327.3
• UniProt Accession #: Q03395; B2R978
• Molecular Weight: 37,205 Da
• NCBI Official Full Name: rod outer segment membrane protein 1
• NCBI Official Synonym Full Names: retinal outer segment membrane protein 1
• NCBI Official Symbol: ROM1
• NCBI Official Synonym Symbols: ROM; RP7; ROSP1; TSPAN23
• NCBI Protein Information: rod outer segment membrane protein 1
• UniProt Protein Name: Rod outer segment membrane protein 1
• Protein Family: Rhomboid-like protease
• UniProt Gene Name: ROM1
• UniProt Synonym Gene Names: TSPAN23; ROSP1; Tspan-23
• UniProt Entry Name: ROM1_HUMAN

NCBI Description

This gene is a member of a photoreceptor-specific gene family and encodes an integral membrane protein found in the photoreceptor disk rim of the eye. This protein can form homodimers or can heterodimerize with another photoreceptor, retinal degeneration slow (RDS). It is essential for disk morphogenesis, and may also function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Certain defects in this gene have been associated with the degenerative eye disease retinitis pigmentosa. [provided by RefSeq, Jul 2008]

Uniprot Description

ROM1: May function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis. Defects in ROM1 may cause retinitis pigmentosa (RP); when associated with defects in PRPH2. Belongs to the PRPH2/ROM1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane

Biological Process: visual perception; regulation of gene expression; cell adhesion

Disease: Retinitis Pigmentosa; Retinitis Pigmentosa 7