RNF4 sirna

RNF4 siRNA (Human)

Cat. Num. AAA8208169

• Gene Names: RNF4; SLX5; SNURF; RES4-26
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF4; RNF4 siRNA (Human); SNURF; E3 ubiquitin-protein ligase RNF4; RING finger protein 4; Small nuclear ring finger protein; Protein SNURF; RNF4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 190

• Applicable Applications for RNF4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF4 sirna

siRNA to inhibit RNF4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 297139777
• NCBI GeneID: 6047
• NCBI Accession #: NP_001171938.1
• NCBI GenBank Nucleotide #: NM_001185009.2
• UniProt Accession #: P78317; Q49AR8; B2R6D6; D6RF58
• Molecular Weight: 12,126 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF4 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 4
• NCBI Official Symbol: RNF4
• NCBI Official Synonym Symbols: SLX5; SNURF; RES4-26
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF4
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF4
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF4
• UniProt Synonym Gene Names: SNURF; Protein SNURF
• UniProt Entry Name: RNF4_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010]

Uniprot Description

RNF4: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation.

Protein type: Nuclear receptor co-regulator; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Transcription factor

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: PML body; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; androgen receptor binding; zinc ion binding; SUMO polymer binding; transcription coactivator activity; nucleosome binding; ubiquitin-protein ligase activity; transcription factor activity; transcription factor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; transcription, DNA-dependent; response to arsenic; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter