RHOU sirna

RHOU siRNA (Human)

Cat. Num. AAA8210076

• Gene Names: RHOU; ARHU; G28K; WRCH1; hG28K; CDC42L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RHOU; RHOU siRNA (Human); ARHU; CDC42L1; G28K; WRCH1; Rho-related GTP-binding protein RhoU; CDC42-like GTPase 1; GTP-binding protein-like 1; Rho GTPase-like protein ARHU; Ryu GTPase; Wnt-1 responsive Cdc42 homolog 1; WRCH-1; RHOU sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RHOU siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 258

• Applicable Applications for RHOU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RHOU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RHOU sirna

siRNA to inhibit RHOU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11034843
• NCBI GeneID: 58480
• NCBI Accession #: NP_067028.1
• NCBI GenBank Nucleotide #: NM_021205.5
• UniProt Accession #: Q7L0Q8; Q59FE9; Q8TDQ2; B1AKN1
• Molecular Weight: 22,164 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoU
• NCBI Official Synonym Full Names: ras homolog family member U
• NCBI Official Symbol: RHOU
• NCBI Official Synonym Symbols: ARHU; G28K; WRCH1; hG28K; CDC42L1
• NCBI Protein Information: rho-related GTP-binding protein RhoU
• UniProt Protein Name: Rho-related GTP-binding protein RhoU
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOU
• UniProt Synonym Gene Names: WRCH-1
• UniProt Entry Name: RHOU_HUMAN

NCBI Description

This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]

Uniprot Description

RHOU: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP- bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 1q42.11-q42.3

Cellular Component: Golgi membrane; cell projection; focal adhesion; plasma membrane; podosome; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; metal ion binding

Biological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; Rac protein signal transduction; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; G1/S transition of mitotic cell cycle