RFX4 sirna

RFX4 siRNA (Human)

Cat. Num. AAA8213776

• Gene Names: RFX4; NYD-SP10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RFX4; RFX4 siRNA (Human); Transcription factor RFX4; Regulatory factor X 4; Testis development protein NYD-SP10; RFX4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RFX4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 744

• Applicable Applications for RFX4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RFX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RFX4 sirna

siRNA to inhibit RFX4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 331999966
• NCBI GeneID: 5992
• NCBI Accession #: NP_001193620.1
• NCBI GenBank Nucleotide #: NM_001206691.1
• UniProt Accession #: Q33E94; Q33DW6; Q33DW7; Q33E95; Q6YM53; Q8MHQ1; Q8NC78; Q8NDF9; Q8SNA1; Q96S80; A8K5Y0; B2RDW4
• Molecular Weight: 64,181 Da
• NCBI Official Full Name: transcription factor RFX4 isoform d
• NCBI Official Synonym Full Names: regulatory factor X, 4 (influences HLA class II expression)
• NCBI Official Symbol: RFX4
• NCBI Official Synonym Symbols: NYD-SP10
• NCBI Protein Information: transcription factor RFX4
• UniProt Protein Name: Transcription factor RFX4
• Protein Family: Transcription factor
• UniProt Gene Name: RFX4
• UniProt Entry Name: RFX4_HUMAN

NCBI Description

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

Uniprot Description

RFX4: May activate transcription by interacting directly with the X-box. Belongs to the RFX family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: dorsal spinal cord development; regulation of transcription from RNA polymerase II promoter; telencephalon development; cerebellar cortex morphogenesis; transcription, DNA-dependent; midbrain development; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of smoothened signaling pathway in ventral spinal cord patterning