REV3L sirna
REV3L siRNA (Human)
Gene Names
REV3L; POLZ; REV3
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
REV3L; REV3L siRNA (Human); POLZ; REV3; DNA polymerase zeta catalytic subunit; Protein reversionless 3-like; REV3-like; hREV3; REV3L sirna
Host
Synthetic
Reactivity
Human
Specificity
REV3L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
3052
Applicable Applications for REV3L sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human REV3L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for REV3L sirna
siRNA to inhibit REV3L expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
344,154 Da
NCBI Official Full Name
DNA polymerase zeta catalytic subunit isoform b
NCBI Official Synonym Full Names
REV3-like, polymerase (DNA directed), zeta, catalytic subunit
NCBI Official Symbol
REV3L
NCBI Official Synonym Symbols
POLZ; REV3
NCBI Protein Information
DNA polymerase zeta catalytic subunit
UniProt Protein Name
DNA polymerase zeta catalytic subunit
UniProt Gene Name
REV3L
UniProt Synonym Gene Names
POLZ; REV3; REV3-like; hREV3
UniProt Entry Name
DPOLZ_HUMAN
Uniprot Description
REV3: the DNA polymerase zeta catalytic subunit. Catalyzes the production of N diphosphate + {DNA}N from N deoxynucleoside triphosphate. Involved in translesion synthesis and required for the production of UV- and other DNA fork-blocking-induced mutations, as well as the majority of spontaneous mutations.
Protein type: EC 2.7.7.7; DNA replication; Transferase
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleoplasm; zeta DNA polymerase complex; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; DNA-directed DNA polymerase activity; nucleotide binding
Biological Process: error-prone postreplication DNA repair; DNA repair; DNA-dependent DNA replication
Research Articles on REV3L
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Product Notes
The REV3L rev3l (Catalog #AAA829211) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The REV3L siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's REV3L can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the REV3L rev3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REV3L, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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