RDH12 sirna

RDH12 siRNA (Human)

Cat. Num. AAA8226234

• Gene Names: RDH12; LCA3; RP53; LCA13; SDR7C2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RDH12; RDH12 siRNA (Human); Retinol dehydrogenase 12; All-trans and 9-cis retinol dehydrogenase; RDH12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RDH12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 316

• Applicable Applications for RDH12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RDH12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RDH12 sirna

siRNA to inhibit RDH12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 186928839
• NCBI GeneID: 145226
• NCBI Accession #: NP_689656.2
• NCBI GenBank Nucleotide #: NM_152443.2
• UniProt Accession #: Q96NR8; Q8TAW6; B2RDA2
• Molecular Weight: 35,094 Da
• NCBI Official Full Name: retinol dehydrogenase 12
• NCBI Official Synonym Full Names: retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
• NCBI Official Symbol: RDH12
• NCBI Official Synonym Symbols: LCA3; RP53; LCA13; SDR7C2
• NCBI Protein Information: retinol dehydrogenase 12
• UniProt Protein Name: Retinol dehydrogenase 12
• Protein Family: Retinol dehydrogenase
• UniProt Gene Name: RDH12
• UniProt Synonym Gene Names: SDR7C2
• UniProt Entry Name: RDH12_HUMAN

NCBI Description

The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 13 and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015]

Uniprot Description

RDH12: Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinal reductase. Displays high activity toward 9-cis and all-trans-retinol. Also involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity detected. Might be the key enzyme in the formation of 11-cis-retinal from 11-cis-retinol during regeneration of the cone visual pigments. Defects in RDH12 are the cause of Leber congenital amaurosis type 13 (LCA13). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Defects in RDH12 are the cause of retinitis pigmentosa type 53 (RP53). RP53 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.-

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: intracellular

Molecular Function: protein binding; retinol dehydrogenase activity

Biological Process: phototransduction, visible light; visual perception; retinol metabolic process; photoreceptor cell maintenance; retinoid metabolic process

Disease: Leber Congenital Amaurosis 13