RBPJ sirna

RBPJ siRNA (Human)

Cat. Num. AAA8228854

• Gene Names: RBPJ; SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RBPJ; RBPJ siRNA (Human); IGKJRB; IGKJRB1; RBPJK; RBPSUH; Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30; RBPJ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RBPJ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 500

• Applicable Applications for RBPJ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RBPJ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RBPJ sirna

siRNA to inhibit RBPJ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42560227
• NCBI GeneID: 3516
• NCBI Accession #: NP_005340.2
• NCBI GenBank Nucleotide #: NM_005349.3
• UniProt Accession #: Q06330; Q5XKH9; Q6P1N3; B4DY22
• Molecular Weight: 54,297 Da
• NCBI Official Full Name: recombining binding protein suppressor of hairless isoform 1
• NCBI Official Synonym Full Names: recombination signal binding protein for immunoglobulin kappa J region
• NCBI Official Symbol: RBPJ
• NCBI Official Synonym Symbols: SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
• NCBI Protein Information: recombining binding protein suppressor of hairless
• UniProt Protein Name: Recombining binding protein suppressor of hairless
• Protein Family: RBPJ-interacting and tubulin-associated protein
• UniProt Gene Name: RBPJ
• UniProt Synonym Gene Names: IGKJRB; IGKJRB1; RBPJK; RBPSUH; RBP-J; RBP-JK
• UniProt Entry Name: SUH_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]

Uniprot Description

RBPJ: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Belongs to the Su(H) family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; recombinase activity; protein N-terminus binding; chromatin binding; transcription factor activity

Biological Process: somatic stem cell maintenance; sebaceous gland development; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; negative regulation of cell proliferation; negative regulation of ossification; angiogenesis; regulation of timing of cell differentiation; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; epidermal cell fate specification; hair follicle maturation; humoral immune response; activation of Notch receptor target transcription factor; keratinocyte differentiation; inflammatory response to antigenic stimulus; pituitary gland development; B cell differentiation; defense response to bacterium; epithelial to mesenchymal transition; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent; auditory receptor cell fate commitment

Disease: Adams-oliver Syndrome 3