RASA4 sirna

RASA4 siRNA (Human)

Cat. Num. AAA8230420

• Gene Names: RASA4; GAPL; CAPRI
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RASA4; RASA4 siRNA (Human); CAPRI; GAPL; KIAA0538; Ras GTPase-activating protein 4; Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2; RASA4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RASA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 757

• Applicable Applications for RASA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RASA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RASA4 sirna

siRNA to inhibit RASA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 299829175
• NCBI GeneID: 10156
• NCBI Accession #: NP_001073346.2
• NCBI GenBank Nucleotide #: NM_001079877.2
• UniProt Accession #: O43374; O60286; Q14CQ4; Q86UW3; Q96QU0
• Molecular Weight: 85,409 Da
• NCBI Official Full Name: ras GTPase-activating protein 4 isoform 2
• NCBI Official Synonym Full Names: RAS p21 protein activator 4
• NCBI Official Symbol: RASA4
• NCBI Official Synonym Symbols: GAPL; CAPRI
• NCBI Protein Information: ras GTPase-activating protein 4
• UniProt Protein Name: Ras GTPase-activating protein 4
• Protein Family: Ras GTPase-activating protein
• UniProt Gene Name: RASA4
• UniProt Synonym Gene Names: CAPRI; GAPL; KIAA0538
• UniProt Entry Name: RASL2_HUMAN

NCBI Description

This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RASA4: Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: metal ion binding; GTPase activator activity

Biological Process: negative regulation of Ras protein signal transduction