RAP1A sirna

RAP1A siRNA (Human)

Cat. Num. AAA8200545

• Gene Names: RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAP1A; RAP1A siRNA (Human); KREV1; Ras-related protein Rap-1A; C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1; RAP1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAP1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 184

• Applicable Applications for RAP1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAP1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAP1A sirna

siRNA to inhibit RAP1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58331202
• NCBI GeneID: 5906
• NCBI Accession #: NP_001010935.1
• NCBI GenBank Nucleotide #: NM_001010935.2
• UniProt Accession #: P62834; P10113
• Molecular Weight: 20,987 Da
• NCBI Official Full Name: ras-related protein Rap-1A
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A
• UniProt Protein Name: Ras-related protein Rap-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1
• UniProt Entry Name: RAP1A_HUMAN

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.

Protein type: G protein; Motility/polarity/chemotaxis; Tumor suppressor; G protein, monomeric; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol

Molecular Function: GTPase activity; protein binding; Rap guanyl-nucleotide exchange factor activity; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity

Biological Process: microvillus biogenesis; platelet activation; protein transport; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction