RANBP2 sirna

RANBP2 siRNA (Human)

Cat. Num. AAA8232772

• Gene Names: RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RANBP2; RANBP2 siRNA (Human); NUP358; E3 SUMO-protein ligase RanBP2; 358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270; RANBP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RANBP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3224

• Applicable Applications for RANBP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RANBP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RANBP2 sirna

siRNA to inhibit RANBP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 150418007
• NCBI GeneID: 5903
• NCBI Accession #: NP_006258.3
• NCBI GenBank Nucleotide #: NM_006267.4
• UniProt Accession #: P49792; Q13074; Q15280; Q53TE2; Q59FH7
• Molecular Weight: 358,199 Da
• NCBI Official Full Name: E3 SUMO-protein ligase RanBP2
• NCBI Official Synonym Full Names: RAN binding protein 2
• NCBI Official Symbol: RANBP2
• NCBI Official Synonym Symbols: ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
• NCBI Protein Information: E3 SUMO-protein ligase RanBP2
• UniProt Protein Name: E3 SUMO-protein ligase RanBP2
• Protein Family: RanBP2-type zinc finger protein
• UniProt Gene Name: RANBP2
• UniProt Synonym Gene Names: NUP358; RanBP2
• UniProt Entry Name: RBP2_HUMAN

NCBI Description

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008]

Uniprot Description

RANBP2: a very large RAN-binding nucleoporin with E3 SUMO-protein ligase activity. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Forms a complex with SUMOylated RanGAP1 (the GTPase activating protein for Ran) that is required for efficient importin alpha/beta dependent nuclear import. May bind RNA or DNA. Component of the nuclear export pathway. Has a specific docking site for the nuclear export factor exportin-1. Contains a cyclophilin type peptidyl-prolyl cis-trans isomerase domain. A giant scaffolding protein that forms filaments extending from the nuclear pore into the cytoplasm. Regulates the localization of mitochondria in photosensory neurons. Deficits of RanBP2 downregulate glucose catabolism and energy homeostasis. Forms a tight complex with RANBP1 and UBE2I. Interacts with SUMO1 and PARK2. For some substrates, sumoylation and nuclear import are linked events. Sumoylates proteins including SP100 and HDAC4 deacetylase.

Protein type: Nucleoporin; Adaptor/scaffold; SUMO conjugating system; EC 5.2.1.8; Nuclear export; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 2q12.3

Cellular Component: nuclear membrane; mitochondrion; membrane; nuclear pore; cytosol; nuclear inclusion body

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; zinc ion binding; RNA binding; Ran GTPase binding; ligase activity

Biological Process: mRNA transport; protein peptidyl-prolyl isomerization; viral reproduction; protein folding; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; negative regulation of glucokinase activity; protein sumoylation; protein import into nucleus; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport

Disease: Encephalopathy, Acute, Infection-induced, Susceptibility To, 3