RABGEF1 sirna

RABGEF1 siRNA (Human)

Cat. Num. AAA8215587

• Gene Names: RABGEF1; RAP1; RABEX5; rabex-5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RABGEF1; RABGEF1 siRNA (Human); RABEX5; Rab5 GDP/GTP exchange factor; RAP1; Rabaptin-5-associated exchange factor for Rab5; Rabex-5; RABGEF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RABGEF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 299

• Applicable Applications for RABGEF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RABGEF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RABGEF1 sirna

siRNA to inhibit RABGEF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 559098403
• NCBI GeneID: 27342
• NCBI Accession #: NP_001273989.1
• NCBI GenBank Nucleotide #: NM_001287060.1
• UniProt Accession #: Q9UJ41; Q3HKR2; Q3HKR3; Q53FG0; B4DZM7
• Molecular Weight: 58,526 Da
• NCBI Official Full Name: rab5 GDP/GTP exchange factor isoform a
• NCBI Official Synonym Full Names: RAB guanine nucleotide exchange factor (GEF) 1
• NCBI Official Symbol: RABGEF1
• NCBI Official Synonym Symbols: RAP1; RABEX5; rabex-5
• NCBI Protein Information: rab5 GDP/GTP exchange factor
• UniProt Protein Name: Rab5 GDP/GTP exchange factor
• Protein Family: Rab5 GDP/GTP exchange factor
• UniProt Gene Name: RABGEF1
• UniProt Synonym Gene Names: RABEX5
• UniProt Entry Name: RABX5_HUMAN

NCBI Description

RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]

Uniprot Description

RABGEF1: Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs, Rab

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: recycling endosome; early endosome; vesicle

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding

Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of inflammatory response; negative regulation of mast cell degranulation; protein ubiquitination; negative regulation of Ras protein signal transduction; endocytosis; negative regulation of receptor-mediated endocytosis; protein targeting to membrane; negative regulation of leukocyte migration