QTRTD1 sirna

QTRTD1 siRNA (Human)

Cat. Num. AAA8200721

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: QTRTD1; QTRTD1 siRNA (Human); Queuine tRNA-ribosyltransferase subunit QTRTD1; Queuine tRNA-ribosyltransferase domain-containing protein 1; QTRTD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: QTRTD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 427

• Applicable Applications for QTRTD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human QTRTD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for QTRTD1 sirna

siRNA to inhibit QTRTD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 378786662
• NCBI GeneID: 79691
• NCBI Accession #: NP_001243764.1
• NCBI GenBank Nucleotide #: NM_001256835.1
• UniProt Accession #: Q9H974; Q6IA59; A6NGE9; B7Z472; B7Z5R2; J3KR78
• Molecular Weight: 48,203 Da
• NCBI Official Full Name: queuine tRNA-ribosyltransferase subunit QTRTD1 isoform 2
• NCBI Official Synonym Full Names: queuine tRNA-ribosyltransferase domain containing 1
• NCBI Official Symbol: QTRTD1
• NCBI Protein Information: queuine tRNA-ribosyltransferase subunit QTRTD1
• UniProt Protein Name: Queuine tRNA-ribosyltransferase subunit QTRTD1
• UniProt Gene Name: QTRTD1
• UniProt Entry Name: QTRD1_HUMAN

NCBI Description

This gene encodes a subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine. The encoded protein may play a role in the queuosine 5'-monophosphate salvage pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

QTRTD1: Interacts with QTRT1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine). Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

Protein type: EC 2.4.2.29

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: mitochondrion; cytoplasm

Molecular Function: zinc ion binding; queuine tRNA-ribosyltransferase activity

Biological Process: queuosine biosynthetic process