PVRL2 sirna

PVRL2 siRNA (Human)

Cat. Num. AAA8218818

• Gene Names: PVRL2; HVEB; PRR2; CD112; PVRR2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PVRL2; PVRL2 siRNA (Human); HVEB; PRR2; Poliovirus receptor-related protein 2; Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin-2; CD antigen CD112; PVRL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PVRL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 538

• Applicable Applications for PVRL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PVRL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PVRL2 sirna

siRNA to inhibit PVRL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 112789532
• NCBI GeneID: 5819
• NCBI Accession #: NP_001036189.1
• NCBI GenBank Nucleotide #: NM_001042724.1
• UniProt Accession #: Q92692; O75455; Q6IBI6; Q96J29; A8K5L5
• Molecular Weight: 51,359 Da
• NCBI Official Full Name: nectin-2 isoform delta
• NCBI Official Synonym Full Names: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• NCBI Official Symbol: PVRL2
• NCBI Official Synonym Symbols: HVEB; PRR2; CD112; PVRR2
• NCBI Protein Information: nectin-2
• UniProt Protein Name: Nectin-2
• UniProt Gene Name: PVRL2
• UniProt Synonym Gene Names: HVEB; PRR2; Herpesvirus entry mediator B; HveB
• UniProt Entry Name: PVRL2_HUMAN

NCBI Description

This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

nectin 2: Probable cell adhesion protein. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cell surface; focal adhesion; plasma membrane; integral to membrane; zonula adherens; intercellular junction

Molecular Function: viral receptor activity; identical protein binding; protein binding; protein homodimerization activity; coreceptor activity; cell adhesion molecule binding

Biological Process: establishment of mitochondrion localization; acrosome formation; intercellular junction assembly and maintenance; regulation of immune response; sperm mitochondrion organization and biogenesis; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; spermatid nuclear differentiation; cytoskeleton organization and biogenesis; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity; virion attachment, binding of host cell surface coreceptor; signal transduction; positive regulation of mast cell activation; fertilization; viral envelope fusion with host membrane; cell part morphogenesis; adhesion to host; homophilic cell adhesion; spermatid development