PVRL1 sirna

PVRL1 siRNA (Human)

Cat. Num. AAA8220531

• Gene Names: PVRL1; ED4; PRR; HIgR; HV1S; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PVRL1; PVRL1 siRNA (Human); HVEC; PRR1; Poliovirus receptor-related protein 1; Herpes virus entry mediator C; Herpesvirus entry mediator C; HveC; Herpesvirus Ig-like receptor; HIgR; Nectin-1; CD antigen CD111; PVRL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PVRL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 517

• Applicable Applications for PVRL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PVRL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PVRL1 sirna

siRNA to inhibit PVRL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42560237
• NCBI GeneID: 5818
• NCBI Accession #: NP_002846.3
• NCBI GenBank Nucleotide #: NM_002855.4
• UniProt Accession #: Q15223; O75465; Q2M3D3; Q9HBE6; Q9HBW2
• Molecular Weight: 39,150 Da
• NCBI Official Full Name: nectin-1 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• NCBI Official Symbol: PVRL1
• NCBI Official Synonym Symbols: ED4; PRR; HIgR; HV1S; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• NCBI Protein Information: nectin-1
• UniProt Protein Name: Nectin-1
• UniProt Gene Name: PVRL1
• UniProt Synonym Gene Names: HVEC; PRR1; Herpesvirus entry mediator C; HveC; HIgR
• UniProt Entry Name: PVRL1_HUMAN

NCBI Description

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq, Oct 2009]

Uniprot Description

nectin 1: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI); also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails. Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7). Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two- third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: presynaptic membrane; adherens junction; cell-cell adherens junction; membrane; intracellular membrane-bound organelle; axon; plasma membrane; integral to membrane; extracellular region; catenin complex

Molecular Function: viral receptor activity; protein binding; protein homodimerization activity; protein heterodimerization activity; virion binding; coreceptor activity; cell adhesion molecule binding; carbohydrate binding

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; axon guidance; cell-cell adhesion; entry of virus into host cell; regulation of synaptogenesis; retina development in camera-type eye; immune response; lens morphogenesis in camera-type eye; cell adhesion; signal transduction; homophilic cell adhesion; iron ion transport

Disease: Cleft Lip/palate-ectodermal Dysplasia Syndrome