PTPRD sirna

PTPRD siRNA (Human)

Cat. Num. AAA8237092

• Gene Names: PTPRD; HPTP; PTPD; HPTPD; HPTPDELTA; RPTPDELTA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPRD; PTPRD siRNA (Human); Receptor-type tyrosine-protein phosphatase delta; Protein-tyrosine phosphatase delta; R-PTP-delta; PTPRD sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PTPRD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,502

• Applicable Applications for PTPRD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PTPRD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPRD sirna

siRNA to inhibit PTPRD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 283484024
• NCBI GeneID: 5789
• NCBI Accession #: NP_001164496.1
• NCBI GenBank Nucleotide #: NM_001171025.1
• UniProt Accession #: P23468; Q3KPJ0; Q3KPJ1; Q3KPJ2; B1ALA0; F5GWT7
• Molecular Weight: 169,277 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase delta isoform 6
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, D
• NCBI Official Symbol: PTPRD
• NCBI Official Synonym Symbols: HPTP; PTPD; HPTPD; HPTPDELTA; RPTPDELTA
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase delta
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase delta
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRD
• UniProt Synonym Gene Names: Protein-tyrosine phosphatase delta; R-PTP-delta
• UniProt Entry Name: PTPRD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of three Ig-like and eight fibronectin type III-like domains. Studies of the similar genes in chicken and fly suggest the role of this PTP is in promoting neurite growth, and regulating neurons axon guidance. Multiple alternatively spliced transcript variants of this gene have been reported. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jan 2010]

Uniprot Description

PTPRD: is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of three Ig-like and eight fibronectin type III-like domains. Studies of the similar genes in chicken and fly suggest the role of this PTP is in promoting neurite growth, and regulating neurons axon guidance. Multiple alternatively spliced transcript variants of this gene have been reported. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jan 2010]

Protein type: Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 9p23-p24.3

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; cell adhesion molecule binding; receptor binding

Biological Process: heterophilic cell adhesion; neuron differentiation; positive regulation of dendrite morphogenesis; protein amino acid dephosphorylation; transmembrane receptor protein tyrosine phosphatase signaling pathway; phosphate metabolic process