PRUNE sirna

PRUNE siRNA (Human)

Cat. Num. AAA8235282

• Gene Names: PRUNE; DRES17; HTCD37; DRES-17
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRUNE; PRUNE siRNA (Human); Protein prune homolog; hPrune; Drosophila-related expressed sequence 17; DRES-17; DRES17; HTcD37; PRUNE sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRUNE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 271

• Applicable Applications for PRUNE sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRUNE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRUNE sirna

siRNA to inhibit PRUNE expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 737675732
• NCBI GeneID: 58497
• NCBI Accession #: NP_001290158.1
• NCBI GenBank Nucleotide #: NM_001303229.1
• UniProt Accession #: Q86TP1; Q5SZF9; Q659E5; Q6P4E0; Q8N654; Q96JU5; Q9C071; Q9C072; Q9UIV0; B2RCH8; B4DFL7
• Molecular Weight: 24,475 Da
• NCBI Official Full Name: protein prune homolog isoform 2
• NCBI Official Synonym Full Names: prune exopolyphosphatase
• NCBI Official Symbol: PRUNE
• NCBI Official Synonym Symbols: DRES17; HTCD37; DRES-17
• NCBI Protein Information: protein prune homolog
• UniProt Protein Name: Protein prune homolog
• Protein Family: Protein prune
• UniProt Gene Name: PRUNE
• UniProt Synonym Gene Names: hPrune; DRES-17; DRES17
• UniProt Entry Name: PRUNE_HUMAN

NCBI Description

This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

PRUNE: Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. Belongs to the PPase class C family. Prune subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Hydrolase; EC 3.6.1.1

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: focal adhesion; cytoplasm; nucleus

Molecular Function: protein binding; inorganic diphosphatase activity; metal ion binding

Biological Process: metabolic process