PRRX1 sirna

PRRX1 siRNA (Human)

Cat. Num. AAA8202329

• Gene Names: PRRX1; PMX1; PRX1; AGOTC; PHOX1; PRX-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRRX1; PRRX1 siRNA (Human); PMX1; Paired mesoderm homeobox protein 1; Homeobox protein PHOX1; Paired-related homeobox protein 1; PRX-1; PRRX1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRRX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 217

• Applicable Applications for PRRX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRRX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRRX1 sirna

siRNA to inhibit PRRX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5902024
• NCBI GeneID: 5396
• NCBI Accession #: NP_008833.1
• NCBI GenBank Nucleotide #: NM_006902.4
• UniProt Accession #: P54821; O60807; B5BUM7
• Molecular Weight: 24,396 Da
• NCBI Official Full Name: paired mesoderm homeobox protein 1 isoform pmx-1a
• NCBI Official Synonym Full Names: paired related homeobox 1
• NCBI Official Symbol: PRRX1
• NCBI Official Synonym Symbols: PMX1; PRX1; AGOTC; PHOX1; PRX-1
• NCBI Protein Information: paired mesoderm homeobox protein 1
• UniProt Protein Name: Paired mesoderm homeobox protein 1
• Protein Family: Paired mesoderm homeobox protein
• UniProt Gene Name: PRRX1
• UniProt Synonym Gene Names: PMX1; PRX-1
• UniProt Entry Name: PRRX1_HUMAN

NCBI Description

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]

Uniprot Description

PRRX1: Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer. Defects in PRRX1 are the cause of agnathia-otocephaly complex (AGOTC). AGOTC is a rare condition characterized by mandibular hypoplasia or agnathia, ventromedial auricular malposition (melotia) and/or auricular fusion (synotia), and microstomia with oroglossal hypoplasia or aglossia. Holoprosencephaly is the most commonly identified association, but skeletal, genitourinary, and cardiovascular anomalies, and situs inversus have been reported. The disorder is almost always lethal. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: nucleolus; nucleus

Molecular Function: sequence-specific DNA binding; transcription coactivator activity

Biological Process: inner ear morphogenesis; cartilage development; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; artery morphogenesis; middle ear morphogenesis; palate development; embryonic cranial skeleton morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic limb morphogenesis

Disease: Agnathia-otocephaly Complex